Eukaryotic expression and identification of human pSecTag2B-CD226 fusion protein
- VernacularTitle:人pSecTag2B-CD226真核表达载体的构建、表达及初步鉴定
- Author:
Lihua CHEN
;
Xinhai ZHANG
;
Xin XIE
;
Boquan JIN
- Publication Type:Journal Article
- Keywords:
CD226;
Eukaryotic expression;
Fusion protein
- From:
Journal of Medical Postgraduates
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct and express the eukaryotic expression vector of human pSecTag2B-CD226(PTA1).Methods: The gene fragment encoding extracellular region of human CD226 was cloned into the eukaryotic expression vector pSecTag2B.After sequencing,the vector was transfected into COS-7 cells,and the expressed molecule was purified by affinity chromatography.Finally,the product was characterized by ELISA.Results: hCD226-6His was successfully expressed.After purification,the concentration of hCD226-6His was 50?g/ml.Conclusion: Human CD226-6His fusion protein involving the extracellular region of CD226cDNA has been successfully expressed and purified,which helps prepare the ground for further functional studies of this molecule.
