Effect of TNF-? on PPAR?2 mRNA expression in 3T3-L1 adipocytes transfected with the human recombinant adiponectin
- VernacularTitle:肿瘤坏死因子?对人脂联素3T3-L1细胞PPAR?2 mRNA表达的影响
- Author:
Qimei SHE
;
Xianzhong SHI
;
Changjiang WANG
- Publication Type:Journal Article
- Keywords:
Adiponectin;
3T3-L1 adipocytes;
PPAR?2;
TNF-?
- From:
Chinese Journal of Diabetes
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe effect of TNF-? on PPAR?2 mRNA expression in 3T3-L1 cells transfected with human recombinant adiponectin. Methods The 3T3-L1 preadipocytes were transfected with the recombinant plasmid pcDNA3.1+-hADPN.The PPAR?2 mRNA expression was quantitated by semi-quantitative RT-PCR. Results (1)Compared with controls(the 3T3-L1 cells and the 3T3-L1 cells with plasmid), the PPAR-?2 mRNA expression of 3T3-L1 cells with human recombinant adiponectin was higher(P
