Optimization of Conditions of Homologous Gene Quantitative Polymerase Chain Reaction
- VernacularTitle:同源基因定量聚合酶链反应条件的优化
- Author:
Jue WANG
;
Qing HUANG
;
Jia PENG
;
Weiling FU
- Publication Type:Journal Article
- Keywords:
Down′s syndrome;
Homologous gene quantitative polymerase chain reaction;
Non-invasive prenatal diagnosis
- From:
Chinese Journal of Nosocomiology
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To evaluate the standard of non-invasive diagnosis of Down′s syndrome between the different PCR machines. METHODS Two kinds of PCR machines were used respectively to amplify the homologous genes-the human liver-like phosphofructokinase gene (PFKL) and the human muscle-like phosphofructokinase gene (PFKM). RESULTS The best annealing temperature of the two PCR machines were 64 ℃ and 60 ℃,and only in this reaction conditions PFKL′s and PFKM′s electrophoresis strips had the same optical density value. CONCLUSIONS This approach has proven that the conditions of amplified the PFKL and PFKM are different between the two kinds of PCR machines.
