Detecting 43Codon of High Mutation Site in rpsL of STR Resistant MTB by Molecular Beacons Technology:An Experimental Study
- VernacularTitle:耐链霉素结核分枝杆菌rpsL基因高突变位点43Codon分子信标检测的实验研究
- Author:
Qinghai CHEN
;
Zhiheng BIAN
;
Hong KUANG
;
Weiling FU
- Publication Type:Journal Article
- Keywords:
STR resistant rpsL gene;
43 Codon of mutation site;
Molecular beacon probe;
Fluorescence microscope
- From:
Chinese Journal of Nosocomiology
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To design molecular beacon probe of 43 Codon of high mutation site in rpsL of STR resistant MTB and its amplification system, meanwhile, to find the way of detecting the fluorescent light and making a qualitative judgment by use of fluorescence microscope and image analysis software. METHODS The software, Beacon designer, was used to design the 43 Codon molecular beacon probe and the amplification system, and the fluorescence microscope and image analysis software were used to detect the fluorescent light and make a qualitative judgment. RESULTS The strap of amplification product was shown clearly. The difference between PCR products from standard strain and STR resistant one was obvious in detecting the fluorescent light by use of fluorescence microscope. The rate of resistant STR detected was about 80% (P
