The Impact of Different Iodine Intakes on Type Ⅰ Iodothyronine Deiodinase Activity and mRNA Expression in Mouse Thyroid Tissue
- VernacularTitle:不同碘摄入水平对小鼠甲状腺组织Ⅰ型脱碘酶基因表达及酶活性的影响
- Author:
Kun WANG
;
Yina SUN
;
Jiayu LIU
;
Yuqin YAN
;
Zupei CHEN
- Publication Type:Journal Article
- Keywords:
type Ⅰ iodothyronine deiodinase, thyroid hormone, gene expression, thyroid function
- From:
Progress in Biochemistry and Biophysics
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Thyroid function ultimately depends on appropriate iodine supply to the gland. Thyroid hormone deiodination is an intrinsic component of the thyroid hormone homeostasis. Type Ⅰ iodothyronine deiodinase (D1) plays an important role in thyroid hormone metabolism and has close relationship with thyroid function. Based on successfully establishing animal models of iodine deficiency and iodine excess in Babl/c mice (Babl/c mice were randomly divided into five groups: low iodine (LI), normal iodine (NI), five-fold iodine (5HI) , ten-fold iodine (10HI) and fifty-fold iodine (50HI) group. Three months and six months after admistration, they were sacrificed and thyroids were excised), the expression level of D1 mRNA were examined by using real time quantitative PCR method. D1 activity was analyzed by 125I-rT3 as substrate combined with ion-exchange chromatography. The thyroid hormone was measured with radioimmunoassay method. The data revealed that in the case of iodine deficiency, both D1 mRNA expression and D1 activity was greatly increased(compared with NI groups, P
