Homocysteine downregulates the expression of estrogen receptor alpha in aorta of ovariectomized rats by methylation modification
- VernacularTitle:同型半胱氨酸经甲基化修饰下调去卵巢大鼠主动脉雌激素受体?基因的表达
- Author:
Junxu LIU
;
Jingge ZHANG
- Publication Type:Journal Article
- Keywords:
homocysteine;
estrogen receptor;
methyaltion;
rats
- From:
Basic & Clinical Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of homocysteine on the expression of estrogen receptor alpha(ER?) mRNA and the CpG island methylation of ER? gene promotor region in rats.Methods The serum level of homocysteine in rats was measured by reversed-phase HPLC.RT-PCR and methylated specific PCR(MSP) were applied to assay mRNA expression and the methylation status in promoter CpG island of ER? gene in aorta and cultured rat aortic smooth muscle cells(RASMCs).Results The homocysteine level was increased in ove and ove+H groups(P
