Role of transforming growth factor-?1 inducing myofibroblast formation in hypoxic pulmonary vascular remodeling
- VernacularTitle:TGF-?1诱导成肌纤维细胞形成在低氧性肺血管重塑中的作用
- Author:
Bowan LI
;
Yongliang JIANG
;
Aiguo DAI
- Publication Type:Journal Article
- Keywords:
transforming growth factor-?1;
myofibroblast;
hypoxia;
hypertension;
pulmonary
- From:
Basic & Clinical Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biological effect of transforming growth factor-?1(TGF-?1) on inducing myofibroblast formation in hypoxic pulmonary vascular remodeling.Methods Forty male Wistar rats were exposed to hypoxia for 0,3,7,14 or 21 days.Mean pulmonary arterial pressure(mPAP),vessel morphometry,right ventricle hypertrophy index(RVHI) were measured.Immunocytochemistry was used to measure the expression of ?-Smooth-muscle actin(?-SMA) and TGF-?1 in pulmonary artery walls and in situ hybridization was used to measure the expression of TGF-?1 mRNA in pulmonary artery walls.Ultrastructure alveolar wall vessels were observed by electron microscopy.Human embryonic lung fibroblasts(KMB17) phenotype after induction of hypoxiaand TGF-?1 were recorded through cell culture.Results(1) mPAP increased significantly after 7-day of hypoxia(P
