Decreased phosphorylation of cPKC?-interacting ERK1/2 in the hippocampus of hypoxic preconditioned mice
- VernacularTitle:低氧预适应小鼠脑海马组织内与cPKC?相互作用的ERK1/2磷酸化水平降低
- Author:
Nan ZHANG
;
Song HAN
;
Junfa LI
- Publication Type:Journal Article
- Keywords:
cerebral hypoxic preconditioning;
cPKC?;
ERK1/2;
co-immunoprecipitation
- From:
Basic & Clinical Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the changes in phosphorylation of conventional protein kinase C?(cPKC?)-interacting extracellular signal regulated kinase 1/2(ERK1/2) in the hippocampus of hypoxic preconditioned(HPC) mice.Methods Healthy male BalB/c mice were used to develop "auto-hypoxia"-induced HPC mice and randomly divided into 3 groups as follows: normoxic control(H0),early(H3) and delayed hypoxic preconditioned groups(H6).Co-immunoprecipitation and Western blot were applied to quantitatively analyze the phosphorylation level of cPKC?-interacting ERK1/2 in cytosolic and particulate fractions of hippocampus of HPC mice.ResultsERK1/2 was co-immunoprecipitated by cPKC? in hippocampus of mice.The phosphorylation level of cPKC? interacting ERK1/2 at tyrosine 204 decreased significantly in the hippocampus of H3 and H6 groups as compared with that of the H0 group(P
