Transcription factors FoxO3a expression on adipose tissue of KKay diabetic mice and the effects of treatment with rosiglitazone and metformin
- VernacularTitle:罗格列酮和二甲双胍对小鼠脂肪组织转录因子FoxO3a蛋白表达的影响
- Author:
Yuxiu LI
;
Jingbo ZENG
;
Qi SUN
;
Heng WANG
- Publication Type:Journal Article
- Keywords:
KKay diabetic mice;
FoxO3a;
rosiglitazone;
metformin
- From:
Basic & Clinical Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of FoxO3a in adipose tissue from KKay diabetic mice and the effects of treatment with rosiglitazone and metformin on FoxO3a expression in adipose tissue in order to understand the mechanism of insulin resistance.Methods Mice were randomly divided into 3 groups: the group without any treatment,group with rosiglitazone and group treated with metformin 3 g/kg/day.Control group consists of 7 C57BL mice 16.Dispatched the mice and sampled adipose tissue to measure the protein concentration by Bradford method and to detect Foxo3a protein expression on adipose tissue by Western Blot with multiple colony antibody 1∶1250.Results Foxo3a was highly expressed in adipose from KKAy diabetic mice as compared with that in control group(FoxO3a/?-actin ratio 1.76?0.19 vs 1.15?0.10,P
