Inhibitory effect of antisense oligodeoxynucleotide targeting survivin on growth of human gastric cancer cells
- VernacularTitle:Survivin反义寡聚脱氧核苷酸对人胃癌细胞生长的抑制
- Author:
Lantao XU
;
Yu CUI
- Publication Type:Journal Article
- Keywords:
survivin gene;
antisense oligodeoxynucleotide;
gastric cancer cell;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect of antisense oligodeoxynucleotide (ASODN) targeting survivin gene on gastric cancer cell line SGC-7901. Methods: Cultured SGC-7901 cells were divided into 6 groups: control group, lipofectin group, oligodeoxynucleotide(ODN) group, 1?10-5mol/L ASODN group, 3?10-5mol/L ASODN group and 5?10-5mol/L ASODN group. After incubated with ASODN, the expression of survivin mRNA in the SGC-7901 cells was examinated by PCR; the apoptosis of SGC-7901 cells was studied by the morphological observation using inverted microscope, electron microscope, flow cytometry and DNA agarose electrophoresis; telomerase activity was determined using PCR-based TRAP (telomere repeat amplification protocol) assay; MTT assay was used to study the inhibitory effect of ASODN on the proliferation of SGC-7901 cells. Results: ASODN obviously inhibited the expression of survivin mRNA in the SGC-7901 cells. The apoptotic rate of gastric cell was significantly increased after incubated with ASODN ([7.01?0.21]% vs [25.0?0.52]%, P
