Study on bioactive alkaloids ingredient produced by marine actinomyces N331
- VernacularTitle:海洋放线菌N331生物碱活性组分的研究
- Author:
Qinxiong LIN
;
Yun LIU
;
Tao XI
- Publication Type:Journal Article
- Keywords:
marine actinomyces;
alkaloid;
macroporous resin;
antibacterial activity;
cytotoxicity
- From:
Chinese Journal of Marine Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the antibacterial constituents in the fermentation broth of marine actinomyces N331.Methods The antibacterial components were isolated and purified from the fermentation broth of strain N331 by absorption with macroporous resin and silica gel column chromatography,their physiochemical characteristics and bioactivities were preliminarily investigated.Results A crystal ingredient with bioactivities was isolated from the fermentation broth and it was identified as alkaloids which was composed of several compounds with approximate polarity,and its the minimum inhibitory concentration against sensitive and drug-resistant Staphylococcus aureus was 4?g?mL-1,and its half maximal inhibitory concentration(IC50) was 2.0,3.0?g?mL-1 respectively against KB and lung cancer A549 cells,which was equivalent to cisplatin.Conclusion Marine actinomyces N331 can produce the alkaloidal substances with strong antibacterial activity and cytotoxicity.
