Analysis of adrenoceptor agonist-octopamine in fish sauce by HPLC
- VernacularTitle:鱼露中肾上腺素受体激动剂-章鱼胺分析方法的研究
- Author:
Yanping RONG
;
Tingting ZHU
;
Shunsheng CHEN
;
Jianzhong WANG
;
Yinghong QU
- Publication Type:Journal Article
- Keywords:
HPLC;
fish sauce;
octopamine;
tyramine
- From:
Chinese Journal of Marine Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of octopamine in fish sauce.Methods A phenomenes luna C18 column was used.The mobile phase was 0.02?g?mL-1 citric acid-0.02?g?mL-1 sodium dihydrogen phosphate(7∶3,v/v) and detection wavelength was 274 nm.Results The linear range of octopamine was 104.0%,RSD=1.53%.The detection limit was 5.7ng?mL-1.Conclusion This method is simple,rapid and reliable.It could be used for the determination of octopamine and its related substances in fish sauce.The content of octopamine in Engraulis japonicus sauce is 1055?g?mL-1.
