Effect of polypeptide from Chlamys farreri on PI3K/Akt and ASK1-JNK signal transduction pathway in murine thymocytes after UVB radiation
- VernacularTitle:扇贝多肽对UVB辐射小鼠胸腺淋巴细胞PI3K/Akt和ASK1-JNK信号通路的影响
- Author:
Haiying CHEN
;
Yingbin ZHOU
;
Chunbo WANG
;
Wengong YU
;
Xiaoming LAN
- Publication Type:Journal Article
- Keywords:
polypeptide from Chlamys farreri(PCF);
UVB;
thymocyte;
apoptosis;
signal transduction
- From:
Chinese Journal of Marine Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of polypeptide from Chlamys farreri(PCF) on PI3K/Akt and ASK1-JNK signal transduction pathway in thymocytes after ultraviolet B radiation.Methods Murine thymocytes were exposed to UVB radiation.The reactive oxygen species(ROS) level in thymocytes was detected by colormatching.The activation of Akt was investigated by western-blot.ASK1,JNK activation,mitochondria memberine potential and DNA ladder were also investigated after pretreatment with or without PI3K/Akt pathway inhibitor LY294002.Results PCF could activate Akt,inhibit the activation of the ASK1 apoptosis pathway in murine thymocyte radiated by UVB.Conclusion PCF decreased ROS level,increased Akt activity and inducing ASK1 degradation leading to the inhibition of ASK1-JNK induced apoptosis.
