Study of the effect of Astragalus injection on the cell apoptosis of intracerebral hemorrhage in rats
- VernacularTitle:黄芪注射液对脑出血大鼠细胞凋亡影响的研究
- Author:
Dong XU
;
Zhiping HU
;
Yi QIN
- Publication Type:Journal Article
- Keywords:
intracerebral hemorrhage;
apoptosis;
Astragalus injection
- From:
Journal of Clinical Neurology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the rule of cell apoptosis after intracerebral hemorrhage (ICH) in rats, and to analyze the effect of Astragalus injection on the brain tissue water content and cell apoptosis perihematoma.Methods Experimental ICH models of rats were produced in by injection of Collagenase into right caudate nucleus using stereotaxis technique. After operation, 160 SD rats were randomly divided into 4 test groups according to different intervention time (operation meanwhile group, 6 h group,12 h group and 24 h group after operation, 32 rats in each group) and hemorrhagic control group (32 rats). The rats in test groups were treated with Astragalus injection intraperitoneally once a day. All ICH animals were killed at 4 d or 7 d after ICH. 12 rats from the above each group were used to measure brain tissue water content (dry-wet method). The effect of different initiation time point of treatment on brain tissue water content and cell apoptosis perihematoma was observed. The apoptosis perihematoma was detected by TUNEL technique.Results Compared with hemorrhagic control group, brain tissue water contents improved significantly in operation meanwhile group (4 d), 6 h group (both 4 d and 7 d) and 12 h group (4 d)(all P
