Effect of different dose of GM1 on inducing human adipose tissue-derived stromal cells into neural cells
- VernacularTitle:不同剂量的神经节苷脂对人脂肪组织来源的基质细胞分化为神经组织细胞的影响
- Author:
Bin LIU
;
Menghai WU
;
Jinxia ZHANG
- Publication Type:Journal Article
- Keywords:
adipose tissue;
stromal cells;
neural cells;
GM1;
GFAP
- From:
Journal of Clinical Neurology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different dose of GM1 on inducing human adipose tissue-derived stromal cells (ADSCs) into neural cells. Methods Human ADSCs were isolated from human liposuction tissues and cultured in NIM with different dose of GM1, which was used to induce ADSCs into neural cells. The experiment was separated into six groups: control group (?-MEM), Group A (NIM), group B (NIM+50 ?mol/L GM1), group C (NIM+100 ?mol/L GM1), group D (NIM+200 ?mol/L GM1) and group E (NIM+400 ?mol/L GM1). Cells' growth and morphologic changes were observed under invert phase-contrast microscopy. The expressions of Nestin, NSE, MAP2 and GFAP were identified by immunocytochemistry methods.Results (1)ADSCs could differentiate into neural cells in NIM with different dose of GM1. The number of neural cells differentiated from ADSCs increased in a certain period, especially in group E at each time point (all P
