Study of MAPKKK5 gene expression of brain tuissur in patients with cryptogenic refractory temporal lobe epilepsy
- VernacularTitle:隐源性难治性颞叶癫癇患者脑内丝裂原活化蛋白激酶激酶激酶5基因表达的研究
- Author:
Gang ZUO
;
Zhen HONG
;
Wenshi WEI
- Publication Type:Journal Article
- Keywords:
epilepsy,temporal lobe;
gene expression;
MAPKKK5
- From:
Journal of Clinical Neurology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To verify the variation of mitogen-activated protein kinase kinase kinase 5 (MAPKKK5) gene expression in patients with cryptogenic refractory temporal lobe epilepsy (TLE) and to evaluate the possible molecular pathogenesis of intractable TLE. Methods Reverse transcription polymerase chain reaction (RT-PCR) and Western-blot analysis were used to measure the expression alterations of MAPKKK5 mRNA as well as its protein product MAPKKK5 in temporal cortex samples from patients who had undergone temporal lobectomy for intractable epilepsy (n=10). Tissues from 10 subjects who did not have epilepsy served as controls. Results The expression of MAPKKK5 mRNA (1.001?0.321) and its protein MAPKKK5 (0.359?0.299) were significantly increased in epileptic brain compared with the controls (0.648?0.157, 0.137?0.084, respectively) (all P
