Characteristics of liver stem cells induced and differentiated into mature hepatocytes in vivo and in vitro
- VernacularTitle:肝干细胞向成熟肝细胞诱导分化的体内外特点
- Author:
Lei GAO
;
Junqi NIU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Liver stem cells can be induced and differentiated into mature hepatocytes. Transplanted liver stem cells would be good to recovery of damaged hepatic tissues and compensate some hepatic functions. OBJECTIVE: To introduce the research status quo of liver stem cell induced and differentiated into mature hepatocytes in vivo and in vitro RETRIEVAL STRATEGY: We searched in Pubmed for the literatures published from January 2000 to October 2007 with the of "liver stem cell,differentiation"in English. We also searched in Wanfang database for relevant articles published from January 2000 to October 2007 in Chinese. Inclusive criterion:The articles related to the induction and differentiation of liver stem cells were collected. Exclusive criteria:Repetitive investigation or Meta analysis articles were excluded. LITERATURE EVALUATION: From over 100 pieces we chosen 38 pieces of articles which focus on the induction and differentiation of liver stem cells,and those published in the more authorized journals within recent years were superiority. Of the total,5 pieces were related on isolating culture and proliferation of cells,22 pieces on induction and differentiation in vitro,and 11 pieces on induction and differentiation in vivo. From over 30 pieces of articles,we summed up and arranged those about differentiation into mature hepatocytes. Of them,28 were used as reference for review. DATA SYNTHESIS: According to origin, liver stem cells can be divided into liver-derived liver stem cells and non-liver-derived liver stem cells. The former include oval cells,differentiated liver cells,fetal liver cells and bile duct endothelial cells. The latter include embryonic stem cells,bone marrow haemopoietic stem cells,pancreas glandular epithelium cells and enterocytes. All are characterized by pleiotropia variation. Induction and differentiation of liver stem cells:①in vitro:Cell induction culture systems can be different cytokines or chemical agents or patho-microenvironment,and these systems induce liver stem cells into hepatocytes. ② in vivo:Liver stem cells transplanted in body through various kinds of methods would improve liver function and structure. CONCLUSION: The study of liver stem cells is in the period of theory and experiment study. We must strengthen the study of mechanism of its induction and differentiation,prevent stem cells differentiate into tumor cells,and promote it differentiate into mature hepatocytes,so that we can propel liver stem cells in clinical application.
