Preparation of Monoclonal Antibody Against "a" Determinant With Mutation on Site 145 and Rapid Diagnosis Assay for HBV Mutant Strain
- VernacularTitle:“a”决定簇145位点变异的HBsAg单克隆抗体制备及HBV变异株快速检测方法
- Author:
Jun WANG
;
Ji WANG
;
Li WANG
- Publication Type:Journal Article
- Keywords:
hepatitis B virus surface antigen(HBsAg), monoclonal antibody, HBV mutant strain, ELISA
- From:
Progress in Biochemistry and Biophysics
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Hepatitis B virus surface antigen with mutation on site 145 may cause omission in detection of hepatitis B virus with HBsAg diagnostic reagent. Monoclonal antibody against this mutation was obtained through hybridoma technique. The sub-class of the monoclonal antibody was determined to be IgG1(? type, the titer is 1∶9?104), and the monoclonal antibody has no cross-reaction to other hepatitis virus. The method of AC-ELISA which was established with monoclonal antibody and polyclonal antibody showed high analysing susceptibility for HBV mutant samples.
