Effects of lasR/rhlR Mutation on Biofilm Formation of Pseudomonas aeruginosa in vitro
- VernacularTitle:铜绿假单胞菌群体感应系统中lasR/rhlR基因缺陷对生物膜形成的影响
- Author:
Xiaolan LIU
;
Zhijun SONG
;
Jinliang KONG
;
Hong WU
;
Yiqiang CHEN
;
Lizan WANG
;
Hengzhuang WANG
- Publication Type:Journal Article
- Keywords:
Biofilm;
Quorum sensing;
Pseudomonas aeruginosa
- From:
Chinese Journal of Nosocomiology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To understand the effects of lasR/rhlR mutations on the biofilm formation of Pseudomonas aeruginosa in vitro.METHODS The biofilms of PAEO1 and lasR,rhlR,and lasR rhlR mutants were incubated in a polyvinyl chloride tube in 0.9% saline and studied by means of scanning electronic microscopy(SEM) after three days′ incubation.RESULTS After 3 day′s incubation,PAEO1 biofilm showed a well-developed structure,whereas thin and poor developed biofilms were seen in lasR/rhlR mutant groups.CONCLUSIONS lasR/rhlR Genes play probably an important role in the formation of P.aeruginosa biofilm in vitro.
