Detection of Gene Resistant to ?-Lactam, Aminoglycosides and Disinfector among Clinical Isolated Strains of Multi-resistant Pseudomonas aeruginosa
- VernacularTitle:多药耐药铜绿假单胞菌对?-内酰胺类、氨基糖苷类、消毒剂耐药相关基因研究
- Author:
Dong CHANG
;
Wei JIANG
;
Yong YU
;
Heng ZHANG
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Multi-resistance;
Gene
- From:
Chinese Journal of Nosocomiology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the positive rate of ?-lactamase,oprD2,aminoglycosides modifying enzyme and qacE△1 gene among clinical isolated strains of multi-resistant Pseudomonas aeruginosa in our hospital.METHODS P.aeruginosa was determined by VITEK,and MIC was determined by agar dilution method.TEM,IMP,VIM,oprD2,aac(3)-Ⅱ,aac(6′)-Ⅰ,aac(6″)-Ⅱ,ant(3″)-Ⅰ,ant(2″)Ⅰ and qacE△1 in strains were detected by polymerase chain reaction(PCR).RESULTS The positive rate of TEM,aac(3)Ⅱ,aac(6′)-Ⅱ,ant(3″)-Ⅰ and ant(2″)-Ⅰ in multi-resistant P.aeruginosa were 51.4%,48.6%,40.0%,54.3%,45.7% and 60.0%,respectively.No IMP and VIM genes were detected.CONCLUSIONS Positive rate of ?-lactamase, aminoglycosides modifying enzyme is high in 35 tested strains,and that should be paid more attention.
