Effect of SDF-1/CXCR4 on the hematopoietic-supporting activity of bone marrow mesenchymal stem cells
- VernacularTitle:SDF-1/CXCR4增强骨髓间质干细胞的造血支持作用
- Author:
Dongping CHEN
;
Zhijian ZHANG
;
Xiuli WU
;
Jianhua LIN
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
hematopoietic stem/progenitor cells;
stromal cell-derived factor-1;
CXCR4
- From:
Basic & Clinical Medicine
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of stromal cell-derived factor-1(SDF-1)/CXCR4 on the proliferation of CD34+ hematopoietic stem/progenitor cells(HSPCs) supported by bone marrow mesenchymal stem cells(MSCs).Methods Rat bone marrow MSCs were plated as feeder layer in long-term cultures(LTC) with bone marrow CD34+ cells in vitro.Cultures were weekly supplemented with SDF-1,anti-SDF-1 antibody,or anti-CXCR4 antibody separately for 5 weeks.The hematopoietic-supporting activity was evaluated by the number of CD34+ cells and colony forming cell(CFC).To assess the effect of SDF-1/CXCR4 on CD34+ cell proliferation cycle,killing assays were carried out and proliferation indexes were calculated.Expression of SDF-1 and CXCR4 in MSCs and CD34+ cells were determined by flow cytometry.SDF-1 in MSC-and CD34+ cell-conditioned medium was also measured by ELISA.Results The number of CD34+ cells,CFC and proliferation indexes were significantly increased in treat-ment with SDF-1(P
