Optimization for differentiation of P19 cells to cardiomyocytes in vitro
- VernacularTitle:P19细胞心肌体外分化诱导条件的优化
- Author:
Xiaoshan YUE
;
Masato NAGAOKA
;
Toshihiro AKAIKE
;
Zhao WANG
- Publication Type:Journal Article
- Keywords:
P19 cells;
cardiomyocyte;
differentiation
- From:
Basic & Clinical Medicine
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Evaluating important factors which have effects on cardiomyocyte differentiation and to optimize differentiation efficiency.Methods Hanging drop method or 96-well plate method were used to produce aggregates.DMSO was used to induce cardiomyocyte differentiation of P19 cells.Beating cells were detected with microscopy and cardiomyocyte specific protein Troponin T was examined by immunostaining.RT-PCR was applied to evaluate the expression of cardiomyocyte related genes.Results Compared with Hanging drop method,96-well plate method facilitated aggregate formation and adhesion.Adding DMSO improved aggregates integrity.Seeding concentration of 1?107～2?108L-1 caused the most effective differentiation.Troponin T immunostaining was observed before cells started beating.Conclusion By optimizing the method for aggregates formation,differentiation inducing chemicals and cell seeding concentration,it is possible to improve cardiomyocyte differentiation effect of P19 cells.This might provide us a way for cardiomyocyte differentiation with high efficiency.
