RPTC cells toxicity induced by cisplatin and anti-apoptosis mechanism of BCL-2
- VernacularTitle:顺铂诱导肾小管上皮细胞毒性及BCL-2对抗细胞凋亡
- Author:
Leping FENG
;
Wei QIAO
;
Zheng DONG
- Publication Type:Journal Article
- Keywords:
cell apoptosis;
cisplatin;
BCL-2 gene;
signaling transduction
- From:
Basic & Clinical Medicine
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechnism of toxicity induced by cisplatin on renal proximal tubular cells(RPTC) line and anti-apoptosis mechanism of BCL-2 with transfection of different mutant BCL-2 in vitro.MethodsRPTC was translocated with different mutants BCL-2(s).Cell apoptosis induced by cisplatin on RPTC was analyzed with confocal and flurencent microscope.The cell apoptosis was measured with Hoechst33258 after treatment with cisplatin.Results Different mutant BCL-2(BCL-acta,BCL-cb5)were translocated on mitochondrial and Endoplasmic Reticulum(ER) respectively.BCL-acta protected RPTC from apoptosis induced by cisplatin more easily than BCL-cb5 group in a time-dependant manner(P
