Effects of purified product from trapa manshurica fler on apoptosis and cell cycle progression of tumor cells in vitro
- VernacularTitle:3,4,5-三羟基苯甲酸对肿瘤细胞凋亡及细胞周期进程的影响
- Author:
Zhe LU
;
Shouliang GONG
;
Fenglan NIU
;
Xuedong WANG
- Publication Type:Journal Article
- Keywords:
trihydroxybenzoic acid dimmer;
apoptosis;
cell cycle
- From:
Journal of Jilin University(Medicine Edition)
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of 3,4,5-trihydroxybenzoic acid dimmer (TAD) in different doses on apoptosis and cell cycle progression of liver cancer SMMC-7721 cells in vitro and explore its possible mechanism of inhibiting tumor growth.Methods The experiment was divided into 0,3.125,6.250,12.500 and 25.000 ?g TAD groups. Apoptosis was observed with JEM-1200EX transmission electron microscope (TEM).The changes of apoptosis and cell cycle progression were measured with flow cytometry.Results The typical features of apoptosis were found in tumor cells,the nuclei were broken to pieces,mitochondrion cristae were disrupted and vacuoles were showed in nucleus and cytoplasm in 25.000 ?g TAD group observed under TEM.Meantime,the apoptotic percentages of the cells treated with 3.125—25.000 ?g TAD were increased significantly as compared with control group (P
