Constitution of Rat Glomerular Mesangial Cell Stably Expressing Decorin Gene
- VernacularTitle:稳定表达饰胶蛋白聚糖基因的大鼠肾小球系膜细胞株的建立
- Author:
Huijun WANG
;
Zhigang ZHANG
;
Wensheng LIN
;
Qi CHEN
;
Muyi GUO
;
Ping GUANG
- Publication Type:Journal Article
- From:
Fudan University Journal of Medical Sciences
2001;28(2):97-99，102
- CountryChina
- Language:Chinese
-
Abstract:
PurposeTo study the possibility of transferring decorin gene to rat glomerular mesangial cell. Methods Amplification of the rat decorin (DCN) eDNA by RT-PCR for constructing the plasmid pcDNA3.1A-DCN and lipofectin method for transfecting DCN gene into MsC; G418 scanning, Western blot and RT-PCR analysis for detecting DCN protein and mRNA in D-A6 cell clone.Results The recombinant eukaryotic expression plasmid, pcDNA3.1A-DCN was successfully constructed and 2 cell clones positively expressing DCN were selected. ConclusionsThese cell clones positively expressing DCN is valuable for providing favorable experimental bases of gene therapy to model with glomerular diseases.
