Proliferation inhibition of chlorpromazine combined with taxol on Hep-2 cells
- VernacularTitle:氯丙嗪协同紫杉醇对Hep-2细胞的增殖抑制作用
- Author:
Qingjie FENG
;
Yanping XU
;
Ding XIN
;
Zhongying FU
- Publication Type:Journal Article
- Keywords:
chlorpromazine;
taxol;
Hep-2 cell;
cell cycle;
apoptosis
- From:
Journal of Jilin University(Medicine Edition)
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the proliferation inhibition of chlorpromazine combined with taxol on Hep-2 cells (human laryngeal carcinoma cell line) and the effects on cell cycle progression. Methods Hep-2 cells at logarithmic growth phase were divided into taxol groups(3.0,6.0 and 12.0 mg?L-1),chlorpromazine(12.0 mg?L-1) comined with taxol (4 mg?L-1)group and control group (100 mL culture fluid).MTT and flow cytometry were used to detect the proliferation inhibition rates of Hep-2 cells in various groups.Flow cytometry was also used to analyze the cell cycle progression of Hep-2 cells and apoptotic rate after administration.Results The proliferation inhibition rates in 3.0,6.0 and 12.0 mg?L-1 taxol groups were 14.0%,23.9% and 36.7%,respectively,there were significant differences between three groups(P
