Screening affinity peptides of transforming growth factor beta type Ⅱreceptor from the phage display peptide library
- VernacularTitle:噬菌体肽库筛选转化生长因子?Ⅱ型受体亲和肽
- Author:
Yi HU
;
Qinglin WANG
- Publication Type:Journal Article
- Keywords:
Transforming growth factor beta type Ⅱreceptor;
Affinity short peptide;
Phage display 12-mer peptide library;
Hepatic fibrosis
- From:
Journal of Medical Postgraduates
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To screen high-affinity short peptides of the transforming growth factor beta typeⅡ(TGF-? Ⅱ) receptor from the phage display 12-mer peptide library.Methods: The recombinant solube human TGF-? Ⅱ receptor was used as the target to screen its affinity short peptides from the phage display 12-mer peptide library.The positive phage clones were obtained by 3 rounds of biopanning,followed by single stranded DNA extraction and sequence analysis.Results: Four nucleotide sequences were obtained from 10 positive phage clones with high-affinity TGF-? Ⅱ receptor.Neither conservative nor homologous sequence was found by sequence analysis compared with the transforming growth factor beta 1.Conclusion: Phage short peptides have been screened from the phage display library,which helps pave the ground for further researches on affinity short peptides and their role in inhibiting hepatic fibrosis.
