Establishment of Epstein-Barr virus-transformed B cell lines from healthy volunteers of type B
- VernacularTitle:分泌血型特异性抗体人B淋巴细胞系的建立
- Author:
Xiuzhen PAN
;
Jianhua YUE
;
Xianfu LI
;
Changjun WANG
;
Jiaqi TANG
- Publication Type:Journal Article
- Keywords:
Epstein-Barr virus;
Transformation;
B lymphocyte;
Blood type
- From:
Journal of Medical Postgraduates
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop Epstein-Barr virus(EBV)-transformed human peripheral blood B cell lines from healthy volunteers of type B.Methods: B lymphocytes from healthy volunteers of type B capable of producing anti-A antibodies were transformed by EBV,while Cyclosporine A(CsA),as an immunosuppressive agent that could selectively inhibit T-lymphocytes and protect B-lymphocytes from regression,was used in the experiment.Then,the supernanant of the cell culture medium was tested with red blood cells of type A,B and O by agglutinin assay.Results: Twelve of the15 EBV-transformed B lymphocyte cell lines were established,with a success rate of 80%,while 9 human B lymphocyte lines secreted anti-A antibodies.Conclusion: Human B lymphocyte lines secreting antibodies to A antigens were successfully developed,which helps further studies of human blood specific monoclonal antibodies.
