Development of the 2148hk/rr Gene Knock-out Mutant of Streptococcus suis Type 2
- VernacularTitle:Ⅱ型猪链球菌二元信号转导系统2148hk/rr基因敲除突变体的构建
- Author:
Gong CHENG
- Publication Type:Journal Article
- Keywords:
Streptococcus suis type 2;
Two-component signal transduction system;
Gene knock-out;
Mutant
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
To construct gene knock-out mutant of a two-component signal transduction system named 2148hk/rr in Streptococcus suis type 2 virulent strain 05ZYH33.Recombinant gene knock-out vector was constructed consisting of Spc~r cassette with flanking homology regions to the target genes,the isogenic 2148hk/rr-deficient mutant was screened by allelic replacement.PCR analysis and Southern hybridization confirmed that the coding genes of 2148hk/rr were replaced completely by spc~r cassette.Conclusion The mutant of 05ZYH33 2148hk/rr system was successfully constructed,which laid the foundation for farther research on the role of this two-component signal transduction system during infection.
