Genotypes of Meticillin-resistant Staphylococcus aureus
- VernacularTitle:耐甲氧西林金黄色葡萄球菌HVR基因分型
- Author:
Hongxia CAO
;
Zizhong XIONG
;
Zhongxin WANG
;
Yuanhong XU
;
Xu LI
- Publication Type:Journal Article
- Keywords:
Meticillin-resistant Staphylococcus aureus(MRSA);
Genotype;
Resistance
- From:
Chinese Journal of Nosocomiology
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the genotypes of meticillin-resistant Staphylococcus aureus(MRSA)and its resistance.METHODS A total of 73 MRSA clinical isolates were collected in Anhui Province,MIC of sixteen different antibacterial agents against the isolates were determined by agar dilution method.PCR amplified the mec associated hypervariable region(HVR)of MRSA,and the genotypes were classified based on the fragments of amplified products.The correlation of genotypes and antimicrobial resistance was analyzed.RESULTS Seventy three strains of MRSA from Anhui Province were grouped into A,B and C genotypes based on HVR polymorphism,and respectively 17.8%,23.3%,and 58.8%.All strains were sensitive to vancomycin.CONCLUSIONS MRSA is multi-drug resistant and can be divided into 3 genotypes based on the HVR PCR amplified products.HVR PCR method is a rapid and convenient method for molecular epidemiology study of MRSA infections.
