Expression Situation of esp Gene and Analysis of Relativity with Enterococcus faecalis Resistance
- VernacularTitle:esp基因存在与粪肠球菌耐药的相关性分析
- Author:
Wei RUAN
;
Zuosheng YANG
;
Yimou WU
;
Lin REN
;
Manjuan TANG
- Publication Type:Journal Article
- Keywords:
Enterococcus faecalis;
esp Gene;
Antimicrobicals;
Resistance
- From:
Chinese Journal of Nosocomiology
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the expression situation of esp gene and the relativity between esp gene and resistance of Enterococcus faecalis derived-infection.METHODS The E.faecalis esp gene was amplified by PCR,then it was cloned and sequenced;the minimum inhibitory concentration(MIC) of five antimicrobicals against E.faecalis was determined by agar dilution method.RESULTS The positive rate of esp gene in 61 strains of E.faecalis was 42.6%;the positive rate of esp gene in the resistant strains against ampicillin,ciprofloxacin,high-level gentamicin,and erythromycin was 33.3%,54.8%,70.6%,and 51.0%,respectively,and the positive rate of esp gene in the susceptible strains against above 4 antibacterials was 43.6%,30.0%,7.4%,and 8.3%,respectively.CONCLUSIONS Incidence of esp gene does not correlate with the resistance of E.faecalis against ampicillin,but clearly correlate with the resistance of E.faecalis against ciprofloxacin,high-level gentamicin,and erythromycin;esp could become a marker of high-level gentamicin against E.faecalis derived-infection.
