Clinical and Phylogenetic Characteristics of Escherichia coli Urinary Tract Infections.
- Author:
Ji Eun LEE
;
Youn Hee LEE
;
Chan Hee NAM
;
Ga Young KWAK
;
Soo Young LEE
;
Jong Hyun KIM
;
Jae Kyun HUR
;
Jin Han KANG
- Publication Type:Original Article
- Keywords:
Urinary tract infections;
Escherichia coli;
Phylogenetic groups;
Infants
- MeSH:
Epidemiologic Studies;
Escherichia;
Escherichia coli;
Female;
Humans;
Infant;
Iron;
Male;
Multiplex Polymerase Chain Reaction;
Sex Ratio;
Urinary Tract;
Urinary Tract Infections
- From:Korean Journal of Pediatric Infectious Diseases
2010;17(1):16-22
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We aimed to investigate the clinical and phylogenetic characteristics of Escherichia coli Urinary Tract Infections (E. coli UTI). METHODS: We enrolled patients with culture-proven E. coli UTI, who were admitted at the study hospital from September 2008 to August 2009. We investigated clinical data of patients with E. coli UTI and characteristics of isolated E. coli strains. The phylogenetic groups were classified using triplex polymerase chain reaction (PCR), and the distribution of nine virulent genes was determined by multiplex PCR. RESULTS: A total of 47 patients have participated in this study. Thirty (63.8%) were under 6 months; eight (17.0%) were between 6-12 months; and nine (19.1%) were over 12 months. We compared two age groups between under 6-month and over 6-month. In the age group under 6-month, higher proportion of male (P=0.002) and group B2 strains (P=0.020) were observed. In contrast, higher proportion of female and group non-B2 strains were observed in age group over 6-month. Frequencies of papC, papGII, papGIII, sfa/foc, hlyC, cnf1, fyuA, iroN and iucC were estimated as 68.1%, 57.4%, 42.6%, 46.8%, 46.8%, 31.9%, 87.2%, 48.9% and 63.8%, respectively. In the comparison of phylogenetic groups, group B2 showed higher distribution of virulent genes, while group D included more strains resistant to trimethoprim/sulfamethoxazole (TMP/SMZ) than other groups. CONCLUSION: We showed the age group-specific difference in the distribution of sex ratios and phylogenetic groups; more male and group B2 strains in age group under 6-month, while more female and group non-B2 in age group over 6-month. However, further evaluation including larger number of patients will be necessary to confirm above thesis in future molecular epidemiological studies.
