Sphingosine 1-phosphate prolongs action potential duration and inhibits voltage-dependent potassium current in guinea-pig ventricular myocytes
- VernacularTitle:1-磷酸鞘氨醇延长豚鼠心室肌细胞动作电位及抑制电压依赖性钾电流
- Author:
Jingyan GE
;
Wenjie ZHANG
;
Xia CHEN
;
Xiaoxia SUN
;
Ming ZHAO
;
Chunyan ZHAO
;
Guogan ZHONG
- Publication Type:Journal Article
- Keywords:
sphingosine 1-phosphate;
ventricular myocytes;
action potential;
voltage-dependent potassium current
- From:
Basic & Clinical Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of sphingosine 1-phosphate(S1P)on action potential(AP)and voltage-dependent potassium current(KV)in isolated guinea pig ventricular myocytes.Methods The ventricular myocytes were isolated by using Langendorff perfusion method.The AP and KV were recorded by whole cell patch-clamp recording technique.Results S1P prolonged the 50% and 90% action potential duration(APD50 and APD90)and decreased KV which were blocked by pertussis toxin(PTX)or Calphostin C.Conclusion S1P decreased KV in a PKC pathway and prolonged action potential duration in guinea-pig ventricular myocytes.
