Effects of norepinephrine on hepatic stellate cells apoptosis in vitro
- VernacularTitle:去甲肾上腺素对体外肝星状细胞系凋亡的影响
- Author:
Na LIU
;
Xiaolan ZHANG
;
Dongmei YAO
;
Xiaopeng TIAN
- Publication Type:Journal Article
- Keywords:
hepatic stellate cell;
NE;
adrenoreceptor;
proliferation;
apoptosis
- From:
Basic & Clinical Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of NE on hepatic stellate cells(HSCs)apoptosis.Methods After NE and the antagonists of adrenoceptor subtypes were administered to HSCs,MTT method was used to evaluate cell proliferation.TUNEL assay and flow cytometry(FCM)were used to identify cell apoptosis.Results(1)Compared with control group,NE at concentrations of 1、10 and 100 ?mol/L induced HSCs proliferation in a time-dependent manner;The action of NE at concentrations of 10 ?mol/L reached the peak(0.262?0.085 vs 0.048?0.055,P
