Comparison between two isolation methods of human mesenchymal stem cells in vitro
- VernacularTitle:两种体外分离成人骨髓间充质干细胞方法的比较
- Author:
Fen YANG
;
Nailong YANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:There is no standard isolation method of mesenchymal stem cells.Percoll density gradient centrifugation is treated as the traditional method among numerous methods but it is so complicated.This study compares whole bone marrow culture to traditional Percoll density gradient centrifugation in order to establish a better method in vitro.METHODS:Experiments were conducted at Central Laboratory of Affiliated Hospital of Qingdao University Medical College from September 2006 to June 2007.Bone marrow was supported by patients who had autologous stem cell transplantation by endocrinology department for diabetes treatment.The experiments had known by the patients and authorized by the hospital ethic committee.The two different methods,whole bone marrow culture and Percoll density gradient centrifugation,were compared in the number of cell clone,cell morphology,cell superficial mark,the differentiation into fat cell.RESULTS:① The number of clone cell by whole bone marrow culture was more than by Percoll density gradient centrifugation(P
