Irritation of ultrahigh molecular weight polyethylene fiber to oral mucous membrane of golden hamsters
- VernacularTitle:超高分子量聚乙烯纤维对金黄地鼠口腔黏膜的刺激实验
- Author:
Xiaoxia QIU
;
Tao WANG
;
Sanxin MO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2007;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To evaluate Ribbond ultrahigh molecular weight polyethylene fiber (UMWPF) on hamster's pouch by oral membrane irritation. METHODS: Experiments were performed at the Animal Experimental Center of Henan Province from July to December 2004. Ten healthy male golden hamsters aged 60-70 days were selected. The samples of UMWPF, positive and negative control group (n=10, diameter 4 mm, thickness 1 mm) were fixed on the oral membrane of hamster's pouch with medical thread. The thread should pass through membrane and fixed on the animals' skin. The experimental samples were on the one side of pouch membrane (UMWPF toward membrane); the positive and negative samples were on the cephalic and tail of the other side respectively. After two weeks' systemic and local observation, the local oral membrane specimens were viewed under microscope. RESULTS: Totally 10 hamsters were included in the final analysis. ①2 weeks after the surgery, the animals pouch membrane contacting with the experiment and negative samples showed no hyperemia, hydrops, erosion or ulcer, and where positive samples contacted showed rough; 7 of them showed light hyperemia and hydrops. ②The epithelia layer of the animal membrane contacting with experiment and negative samples were complete, which cells were in order, epithelial processes stretched to the basal layer. There was no inflammatory cells infiltration, congestion or edema in the connective tissue, but which showed contrast in the positive group. ③The epithelial cells of the experiment and negative groups had no changes in morphology, while in the positive group a few cells swelled and their nucleolus were more clear than normal, and their shapes were circle which was different from normal. There were no systemic or local abnormal response to UMWPF and that the histopathological findings were also normal. CONCLUSION: Ribbond ultrahigh molecular weight polyethylene fiber has no abnormal irritating response to oral membrane of golden hamster.
