Bcl-XL siRNA sensitizes ovary cancer cells in TRAIL-induced apoptosis
- VernacularTitle:Bcl-XL siRNA在TRAIL杀伤卵巢癌细胞中的增敏作用
- Author:
Jingzi HU
;
Hongbo ZHU
;
Xuefeng HUANG
;
Chao HE
- Publication Type:Journal Article
- Keywords:
Bcl-XL small interference RNA;
tumor necrosis factor-related apoptosis-inducing ligand;
ovary cancer cells;
drug-resistance;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the role of Bcl-XL siRNA in sensitizing ovary cancer cells for TRAIL-induced apoptosis and the underlying mechanisms.Methods: Western blotting analysis was performed to confirm whether Bcl-XL siRNA could effectively down-regulate Bcl-XL protein after SKOV3 cells were transfected with Bcl-XL siRNA and then the cells were treated with TRAIL.Flow cytometry analysis and cell counting were used for assessment of apoptotic rate and survival rate,respectively.Western blotting analysis was performed to determine the changes of apoptosis-related protein in SKOV3 cells.Results: Compared with control group,Bcl-XL siRNA transfection effectively down-regulated the expression of Bcl-XL protein and suppressed the growth of SKOV3 cells;the suppression peaked at 96 h after transfection,being 43.9% that of the control group(P
