Inhibitory effects of bone sialoprotein monoclonal antibody on bone-seeking breast cancer cells adhering to bone matrix
- VernacularTitle:骨涎蛋白单克隆抗体对亲骨转移乳腺癌细胞与骨基质黏附的抑制
- Author:
Yingjing GONG
;
Jie WANG
;
Huixue SONG
;
Bing XIA
- Publication Type:Journal Article
- Keywords:
bone sialoprotein;
monoclonal antibody;
breast cancer;
bone metastasis;
TGF-?1
- From:
Chinese Journal of Cancer Biotherapy
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the inhibitory effects of anti-bone sialoprotein(BSP) antibody on human breast cancer cells adhering to bone matrix in vitro.Methods: Expression of BSP in 3 different cancer cell lines(bone seeking breast cancer cell MDA-MB-231-BO,lung adenocarcinoma cell SPCA-1 and colon carcinoma cell LOVO)was detected immunohistochemically.Cells adhering test was carried out to investigate the adhering of the 3 different cancer cell lines to mouse bone matrix in vitro and the inhibitory effect of anti-BSP antibody on MDA-MB-231-BO cells adhering to mouse bone matrix;Enzyme-linked immunosorbent assay was carried out for quantitative detection of TGF-?.Results: BSP immunostaining was positive in MDA-MB-231-BO cells and negative in SPCA-1 and Lovo cells.The number of MDA-MB-231-F10 cells adhering to mouse bone matrix was significantly more than SPCA-1 or Lovo cells(P
