Serial Analysis of Gene Expression in Parasitological Research
- VernacularTitle:基因表达系列分析及其在寄生虫学研究中的应用
- Author:
Qiaoli LI
;
Zhiming ZHANG
;
Zhongdong QIAO
- Publication Type:Journal Article
- Keywords:
SAGE;
mRNA profile;
Parasitology
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Serial analysis of gene expression (SAGE) is a powerful high-throughput experimental technique that allows rapid, quantitative analysis of global gene expression in eukaryotic organisms. A short sequence taq (10~14 bp),which is defined by an anchoring enzyme site at a fixed distance from polyA tail, contains sufficient information to iden-tify mRNA transcript from which it originates. The taqs are ligated to obtain concatemers that are cloned into a plasmid vector for sequencing. The identification and abundance of mRNA can be observed through bioinformatics and statistical analysis of a given tag. SAGE is not only applied in obtaining global profile of gene expression in a given cell or tissue, but also help identify a set of specific genes to the cellular conditions by comparing the profiles constructed for a pair of cells that are kept at different conditions. This review covers a general introduction of SAGE, its protocol, meth-odological evolution and applications in parasite biology.
