Antioxidation of Chondrus yendoi and its protective effect on mitochondria
- VernacularTitle:楔形角叉菜的抗氧化及保护线粒体作用
- Author:
Xingtai LI
;
Shumei ZHANG
;
Jiali SHEN
;
Yanying WANG
- Publication Type:Journal Article
- Keywords:
Chondrus yendoi;
mitochondria;
antioxidant;
superoxide anion
- From:
Chinese Journal of Marine Drugs
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate if Chondrus yendoi decoction(CYD) and polysaccharide(CYP) have the effects of scavenging superoxide anion(O?—2),antioxidation,protecting mitochondria from damage.Methods Lipid peroxidation and swelling of mouse liver and brain mitochondria were induced by Fe2+-L-Cys system in vitro.Superoxide anion was generated by reduced nicotinamide adenine dinucleotide(NADH)-N-methylphenazonium methyl sulfate(PMS)-4-nitrobluetetrazolium chloride(NBT) system.Lipid peroxide malondialdehyde(MDA),mitochondrial swelling and superoxide anion formation were determined by spectrophotometry.Results CYD and CYP could inhibit MDA production,protect mitochondria from swelling,and scavenge O?-2 obviously in a dose-dependent manner.Conclusion CYD and CYP had the effects of scavenging O?-2,antioxidation,protecting mitochondria from injury.CYP was the major bioactive component.
