Folate inhibits methyl-CpG-binding protein 2 expression and cervical cancer cell proliferation
10.3760/cma.j.issn.1006-9801.2012.09.008
- VernacularTitle:叶酸对子宫颈癌细胞甲基-CpG-结合蛋白质2表达及细胞增殖抑制的作用
- Author:
Lan BAI
;
Jintao WANG
;
Ling DING
;
Qian DONG
;
Tingting WU
;
Qin ZHOU
- Publication Type:Journal Article
- Keywords:
Uterine cervical neoplasms;
Folic Acid;
Methyl-CpG-binding protein 2;
Cell proliferation inhibition
- From:
Cancer Research and Clinic
2012;24(9):603-606,609
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the impact of folate on MeCP2 expression and cervical cancer cells growth.Methods Cervical cancer cell lines Caski (HPV16-positive) and C33A (HPV-negative) were treated with different concentrations of folate.MTT,flow cytometry,Western blott and real-time PCR were used to detect the cells’ viability,apoptosis,the expression of MeCP2 protein and mRNA expressions respectively.Results The inhibitions of both cell growth were upgraded with the folate concentration increasing.The differences were significant between the experimental groups and the control group.With increasing of folate concentration,apoptosis ratio of C33A and Caski increased gradually (C33A:r =0.965,P ＜ 0.001; Caski:r =0.973,P ＜ 0.001) and the expression of MeCP2 protein downgraded gradually,presenting significantly negative correlations between them (C33A:r =-0.952,P ＜ 0.001; Caski:r =-0.947,P ＜ 0.001).There was significantly difference for mRNA expression in different concentration groups of Caski and C33A (C33A:F =77.041,P ＜ 0.001; Caski:F =59.885,P ＜ 0.001).In the same concentration group,the expression of MeCP2 protein and mRNA were higher in Caski than that of C33A,and the difference was significant in the concentration of 500 μg/ml group.There was a negative correlation between the expression of MeCP2 protein and cells’ apoptosis ratio (C33A:r =-0.970,P ＜ 0.001; Caski:r =-0.93,P ＜ 0.001).Conclusion Folic acid can inhibit the growth of cerical cancer cells,promote apoptosis and reduce the expression of MeCP2.The aberrant high-expression of MeCP2 can inhibit apoptosis of Caski and C33A.
