The lethal activity of lymphokine activated killer cells from umbilical blood on some human cancer cells
- VernacularTitle:脐血淋巴因子激活的杀伤细胞杀瘤活性的研究
- Author:
Yuedong HE
;
Xiaoling PAN
;
Shanling LIU
- Publication Type:Journal Article
- Keywords:
Cord blood;
Lymphokine activated killer;
Adoptive cellular immunotherapy
- From:
Chinese Journal of Blood Transfusion
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of lymphokine activated killer(LAK) cells induced from cord blood used as adoptive cellular immunotherapy for human cancer.Methods Mononuclear cells were separated from umbilical blood(UBMC) by Ficoll,and stimulated by IL-2.The phenotypes(CD3/ CD4/ CD8) of the mononuclear cells were assayed by Flow cytometry,and their lethal activity on K562 or SKOV6 assayed by MTT colometric.The peripheral blood mononuclear cells were used as the control.Results The in vitro anti-tumor effect of LAK from cord blood was significant.Conclusion LAK from cord blood can be a source of adoptive cellular immunotherapy in the treatment of human cancer.
