Detection of Extended-spectrum ?-Lactamases and AmpC ?-Lactamases and Analysis of Drug Resistance in Enterobacter cloacae
- VernacularTitle:阴沟肠杆菌产超广谱?-内酰胺酶、AmpC酶的检测及耐药性分析
- Author:
Yan LI
;
Shuzhen XU
;
Jianrong SU
;
Zhiyuan LIU
- Publication Type:Journal Article
- Keywords:
Enterobacter cloacae;
?-Lactamases;
Drug resistance
- From:
Chinese Journal of Nosocomiology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the genotypes of extended-spectrum ?-lactamases(ESBLs)and drug resistance in Enterobacter cloacae for clinical treatment.METHODS E.cloacae was identified by VITEK-AMS;ESBLs and AmpC ?-lactamases phenotypes were detected by three-dimensional tests.Detection of ?-lactamases resistant genes were performed by PCR.Plasmid conjugation test was used to determine the location and the transmission of encoding genes of ?-lactamases.The antibotic susceptibility of E.cloacae was detected according to the CLSI guideline.RESULTS The incident rate of ESBLs producing E.cloacae was 48.8% in 86 strains.CTX-M was the main type of ESBLs.The detection rate of CTX-M-3,CTX-M-9,CTX-M-14 and SHV-12 genotypes in isolates were 27.9%,16.3%,3.4% and 18.6%,respectively.Strains producing both ESBLs and AmpC ?-lactamases were 11(12.8%).The strains only producing ESBLs or AmpC ?-lactamases were 31(36.0%) and 8(9.3%),respectively.The sensitive rate of ESBLs isolates to imipenem was 100.0%.Eight ESBLs strains were conjugated successfully.CONCLUSIONS CTX-M and SHV-12 are the main genotypes of ESBLs strains.ESBLs genes can be horizontally transferred to recipient by plasmid conjugated test.
