The construction of pcDNA 3.1 RASSF1 eukaryotic vector and the influence of RASSF1 on apoptosis of hepatocarcinoma cell line

VernacularTitle:真核表达载体pcDNA3.1-RASSF1的构建及其对肝癌细胞凋亡的影响
Author: Yingbin DENG ; Lielie ZHU ; Zhitao LI
Publication Type:Journal Article
Keywords: Ras proteins/GE/ME; Tumor suppressor proteins/GE; Genetic vectors; Liver neoplasms/ ME; Apoptosis
From: Journal of Chinese Physician 2011;13(1):16-18,22
CountryChina
Language:Chinese
Abstract: Objective To construct pcDNA3.1 RASSF1 eukaryotic vector and observe the influence of RASSF1 on the apoptosis of hepatocarcinoma cell line HepG2. Methods RASSF1 gene was amplifled from human RASSF1 cDNA by polymerase chain reaction (PCR) and cloned into pcDNA3.1. The recombinant plasmid pcDNA3. 1 RASSF1 was transfected into hepatocarcinoma HepG2 cell line. The expression of RASSF1 was examined by Western blot. The influence of RASSF1 on the cell apoptosis was measured by Annexin V/PI assay. Results DNA enzyme digestion and sequencing results showed that recombinant plasmid pcDNA3. 1-RASSF1 was successfully constructed. RASSF1 protein was overexpressed in HepG2 cell line transfected with pcDNA3. 1-RASSF1 plasmid. The apoptosis rate of blank, pcDNA3. 1 and pcDNA3. 1-RASSF1 group was (5.8 ±0.42)%, (7.48 ±0.68)% and (35. 1 ±3. 15)%, respectively.Conclusion The pcDNA3. 1- RASSF1 eukaryotic vector was successfully constructed, RASSF1 protein overexpression could induce apoptosis in HepG2 cell line.