Construction of Survivin specific small interfering RNA expression vector,its antiproliferative and proapoptotic effect on lung adenocarcinoma cells
- VernacularTitle:Survivin特异小干扰RNA表达载体构建及对肺腺癌细胞增殖和凋亡的影响
- Author:
Liming LIU
;
Changjie CHEN
;
Zhengxu CHEN
;
Guangjun DUAN
;
Huacheng HU
- Publication Type:Journal Article
- Keywords:
small interference RNA;
Survivin;
neoplasm cell line;
A549 cell
- From:
Basic & Clinical Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test the antiproliferative and proapoptotic effect of Survivin small interfering RNA(siRNA) expression vector on lung adenocarcinoma cell A549 in vitro.Methods The Survivin-siRNA expression vector was constructed and conformed by sequencing.The inhibitory effect of Survivin-siRNA was tested by fluorescent quantitative reverse transcription polymerase chain reaction(FQ RT-PCR),Western blot and immunohistochemistry.The cell proliferation and apoptotic rate were assayed by tetrazolium bromide(MTT)colorimetry and flow cytometry.Results Survivin-siRNA expression vector was constructed and transfected into A549 cell.It effectively reduced mRNA and protein level of Survivin.Immunohistochemistry also showed lower expression of Survivin.The A549 cells transfected with Survivin-siRNA had a lower cellular growth rate than that of control group(P
