Inhibition of anti-PDGF on proliferation of cultured human retinal pigment epithelial cells in vitro
- VernacularTitle:PDGF抗体对体外培养人视网膜色素上皮细胞增生的抑制作用
- Author:
Yazhen WU
;
Hui QI
;
Bin FAN
;
Huiling GUO
;
Fei LI
- Publication Type:Journal Article
- Keywords:
pigment epithelium of eye;
cell culture;
platelet-derived growth factor/immunology
- From:
Journal of Jilin University(Medicine Edition)
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibition of anti-PDGF on proliferation of cultured human retinal pigment epithelial cells(hRPE) in vitro.Methods hRPE were cultivated and were exposed to different concentrations of anti-PDGF(0,1?10-6,5?10-6,1?10-5,5?10-5 and 1?10-4 mg?L-1) respectively .Growth curves were measured with cell counting and the vitalities of cells were examined by percentage of vital cells and total cells.Using MTT staining colorimetric to measure the inhibitory rate.The changes of cell cycle of hRPE were collected and their growth were detected with FCM analysis and the morphological changes of cells were observed by light microscope and electron microscope.Results Anti-PDGF of 1?10-6mg?L-1 stimulated hRPE proliferation slightly.AntiPDGF at dosages ranging from 5?10-6mg?L-1 to 1?10-4mg?L-1 inhibited cell proliferation effectively in a dose-dependent and time-dependent manner(P
