Inhibition of taurine on myocardial fibrosis and its mechanism
- VernacularTitle:牛磺酸对心肌纤维化的抑制作用及机理
- Author:
Yanchun WANG
;
Hong LI
;
Shijie YANG
- Publication Type:Journal Article
- Keywords:
taurine;
myocardium;
fibroblasts;
cyclin-dependent kinases;
transforming growth factor beta
- From:
Journal of Jilin University(Medicine Edition)
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of taurine(Tau) on the myocardial fibrosis and its mechanism.Methods The rat model of myocardial fibrosis was built by subcutaneous injection of isoprel(Iso).And the rats were divided into control group,model group and taurine group(80,160,320 mg?kg-1?d-1).Hydroxyproline measurment was carried out for detecting collagen quantification in myocardium tissue.Histopathology changes were detected by HE staining.In vitro,the cultured neonatal rat cardiac fibroblasts(CFb) were divided into control group,model group and taurine group(40,80,160mmol?L-1).The proliferation of CFb was induced by angiotensin Ⅱ(AngⅡ) and detected by the thiazoleblue(MTT)colorimetric assay.TGF-?1 level was determined by ELISA technique.The expression of cyclin dependent kinase inhibitor p27 was assessed with flow cytometry.Results In vivo,the contents of hydroxyproline were decreased in Tau(160,320 mg?kg-1?d-1) groups as compared with model group(P
