Effect of icariine on the formations of mesenchymal stem cells of umbilical cord to osteoblasts

VernacularTitle:淫羊藿苷诱导人脐带间充质干细胞分化为成骨细胞的实验研究
Author: Xuejian WU ; Fan ZHANG ; Song YANG
Publication Type:Journal Article
Keywords: Umbilical cord; Mesenchymal stem cells; Icariine; Osteoblasts
From: Chinese Journal of Microsurgery 2011;34(1):47-49,后插5
CountryChina
Language:Chinese
Abstract: Objective To study the biological effect of Icariine on human umbilical cord derived mesenchymal stem cells(huCMSCs) and inducing them to differentiate into osteoblasts. Methods MSCs were isolated and expanded in vitro and their surface antigens of huCMSCs were detected by immunohistochemistry. HuCMSCs were assigned into two groups. In the blank control group, huMSCs were incubated in basic medium. In the experimental group huMSCs were incubated in Icariine medium, The proliferation and differentiation of huMSCs were examined by inverted microscope, Alkaline phosphatase (ALP) staining and the determination of ALP activities. Results HuCMSCs were strongly positive for CD44, and negative for CD34; strongly positive expression for ALP and calcium deposition was detected from the second day to the fourth day in Icariine group; blank control group and Icariine group had significant differences (P ＜ 0.05).Conclusion HuCMSCs can be successfully cultured from the adherent tissue pieces, Icariine enhances proliferation and osteogenic differentiation of huCMSCs and be used as an osteoinductive factor.