Antileukemia immune response activated by interleukin-18 transfected dendritic cells of chronic myelogeous leukemia
- VernacularTitle:转染IL-18基因慢性粒细胞白血病树突状细胞的抗白血病作用
- Author:
Jie LI
;
Yanhua DING
;
Mingbai SHAO
;
Guanjun WANG
;
Ningyi JIN
- Publication Type:Journal Article
- Keywords:
leukemia,myelogeous,chronic;
dendritic cells;
interleukin-18;
antileukemia immune response
- From:
Journal of Jilin University(Medicine Edition)
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the antileukemia immune response of IL-18 gene transfected dendritic cells(DCs) of chronic myelogeous leukemia(CML).Methods DCs were transfected with IL-18 gene by liposomes in CML.The expression of IL-18 in IL-18 transfected DCs was detected.The percentages of CD80+ and CD86+ cells in IL-18 tranfected DCs were determined by FCM.The proliferation of T cell,NK and specific CTL kill activity induced by IL-18 gene transfected DCs were detected.Results The quantity of IL-18 in IL-18 tranfected DCs was(596?34.1)pg/2?106cells/48 h,while the culture medium of mock-transduced DCs and DCs did not secrete detectable levels of IL-18.The percentages of CD80+ and CD86+ cells in IL-18 tranfected DCs were higher than that in mock-transfected DCs(P
