Effects of Enalaprilat on proliferation and nitric oxide synthase-nitric oxide system of neonatal rat cardiac fibroblasts
- VernacularTitle:依那普利拉对新生大鼠心肌成纤维细胞增殖及NOS/NO系统的影响
- Author:
Hongxia SUN
;
Hong LI
;
Shijie YANG
- Publication Type:Journal Article
- Keywords:
enalaprilat;
myocardium;
fibroblasts;
nitric oxide synthase;
nitric oxide
- From:
Journal of Jilin University(Medicine Edition)
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of angiotensin Ⅰ-converting enzyme inhibitor Enalaprilat(Ena) on proliferation and nitric oxide synthase-nitric oxide system of neonatal rat cardiac fibroblasts(CFb)and to probe its anticardiac fibrosis mechanism.Methods The cultured neonatal Wistar rat CFb were divided into control group,model group and three doses of Ena groups.CFb were isolated by trypsin digestion method.MTT colorimetric assay was adopted to evaluate cell proliferation,flow cytometry was used to measure cell cycle,nitric acid reductase method and spectrophotometry were used to detect the NO contents and NOS activity respectively with Ena.Results Ena could inhibit CFb proliferation induced by AngⅡ,there were significant differences of MTT values between model group and Ena groups after treated for 24,48 and 72 h(P
